GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306392/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306392GEOGSEfalseiPSC-Derived Hepatocytes from Patients with MASLD Exhibit Early Mitochondrial DysfunctioniPSCs from 10 MASLD patients and 10 healthy control subjects genotyped for the I148M variant of PNPLA3 were differentiated to iPSC-Heps. Mitochondrial mass and function were assessed under basal culture conditions and following short-term exposure to exogenous palmitate. Outcomes included gene expression, mitochondrial oxygen consumption, ROS production and cellular energy status. iPSC-Heps from MASLD patients exhibited mitochondrial alterations characteristic of their diseased origin. The degree of mitochondrial dysfunction seen in MASLD iPSC-Heps was reminiscent of that described clinically in early MASLD, prior to progression to steatohepatitis. Mitochondrial alterations in MASLD iPSC-Heps occurred independently of PNPLA3 genotype.2026/08/25GSE306392GSM9198749GSM9198759GSM9198758GSM9198769GSM9198768GSM9198757GSM9198767GSM9198755GSM9198766GSM9198754GSM9198753GSM9198764GSM9198763GSM9198752GSM9198762GSM9198751GSM9198750GSM9198761GSM9198760GSM919877021290306392Homo sapiens