{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306476/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306476"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Cytokine gene combination on iPS cell-derived T cells synergistically improves migration, proliferation, and memory formation in solid tumors [ATAC-seq]","description":"T cells show anti-tumor efficacy against solid tumor by infiltration, proliferation, and antigen-specific cytotoxicity. In this study, we investigated various combinations of cytokine gene modifications in chimeric antigen receptor-transfected induced pluripotent stem cell-derived T cells (iCAR-T cells). We found that the combination of interleukin 15 (IL-15) and IL-21 gene modification maintained the infiltration and persistence of iCAR-T cells in tumors and significantly prolonged the survival of a subcutaneous tumor-bearing mouse model. As a novel finding, this combination promoted phosphorylation of STAT1 and enhanced the binding of CXCR3 to its promoter region. Furthermore, iCAR-T cells expressing IL-15 and IL-21 were found to have a gene expression profile similar to primary CD8 CAR-T cells in tumors and to have a memory T-cell-like phenotype. Thus, the IL-15 and IL-21 combination may accelerate the therapeutic efficacy of iCAR-T cells for solid tumor immunotherapies as a substitute of peripheral blood CD8 T cells.","dates":{"publication":"2026/08/19"},"accession":"GSE306476","cross_references":{"GSM":["GSM9201895","GSM9201894","GSM9201888","GSM9201889","GSM9201891","GSM9201890","GSM9201893","GSM9201892"],"GPL":["24676"],"GSE":["306476"],"taxon":["Homo sapiens"],"PMID":["[42127898]"]}}