{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306750"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Spatial organization of pulmonary type 2 inflammation by a macrophage-derived cholesterol metabolite","description":"Effective pulmonary immunity requires the precise spatial organization of immune cells, yet the mechanisms guiding their intratissue positioning during inflammation remain unclear. Here, we identify a cholesterol-derived chemotactic axis that spatially organizes T helper 2 (TH2) cells during fungal-induced pulmonary type 2 inflammation. Inflammation-expanded macrophages expressing cholesterol-25-hydroxylase (CH25H) produce 25-hydroxycholesterol, which is converted into the oxysterol 7α,25-dihydroxycholesterol to attract GPR183-expressing TH2 cells into inflammatory Ly6C+ macrophages, promoting fungal persistence. Disruption of this axis via TH2- specific GPR183 deletion restores type 1 macrophage activation and enhances fungal clearance. Our findings reveal a macrophage-driven, metabolite-based mechanism of immunosuppressive cell positioning in inflamed lung tissue.","dates":{"publication":"2026/05/18"},"accession":"GSE306750","cross_references":{"GSM":["GSM9207214","GSM9207215","GSM9207210","GSM9207211","GSM9207212","GSM9207213"],"GPL":["19057"],"GSE":["306750"],"taxon":["Mus musculus"]}}