{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306859/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306859"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Trem2 activation by renal tubular debris sustains Arg1+ macrophage survival and promotes tubular epithelial repair in renal ischemia–reperfusion injury","description":"Renal ischemia–reperfusion injury (RIRI) profoundly impairs the function of both native and transplanted kidneys. Arg1⁺ macrophages play a pivotal role in tubular regeneration and repair. The myeloid receptor triggering receptor expressed on myeloid cells 2 (Trem2) mediates the clearance of cellular debris by recognizing lipid components from injured cells. This study aimed to investigate whether Trem2 not only facilitates the debris clearance activity of Arg1⁺ macrophages in renal tubules but also sustains their survival, thereby providing a potential therapeutic target for the prevention and treatment of RIRI. To this end, we established stable RAW264.7 cell lines with Trem2 knockdown using lentivirus-mediated shRNA, followed by IL-4–induced polarization. Tubular epithelial fragments were prepared from TCMK-1 cells subjected to hypoxia/reoxygenation (H/R) and subsequently co-cultured with macrophages. RNA sequencing was performed to compare transcriptional profiles between normal Arg1⁺ macrophages and Trem2-deficient Arg1⁺ macrophages under tubular debris stimulation.","dates":{"publication":"2026/04/13"},"accession":"GSE306859","cross_references":{"GSM":["GSM9210935","GSM9210934","GSM9210933","GSM9210932","GSM9210931","GSM9210930"],"GPL":["34290"],"GSE":["306859"],"taxon":["Mus musculus"],"PMID":["[42039193]"]}}