GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306859/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306859GEOGSEfalseTrem2 activation by renal tubular debris sustains Arg1+ macrophage survival and promotes tubular epithelial repair in renal ischemia–reperfusion injuryRenal ischemia–reperfusion injury (RIRI) profoundly impairs the function of both native and transplanted kidneys. Arg1⁺ macrophages play a pivotal role in tubular regeneration and repair. The myeloid receptor triggering receptor expressed on myeloid cells 2 (Trem2) mediates the clearance of cellular debris by recognizing lipid components from injured cells. This study aimed to investigate whether Trem2 not only facilitates the debris clearance activity of Arg1⁺ macrophages in renal tubules but also sustains their survival, thereby providing a potential therapeutic target for the prevention and treatment of RIRI. To this end, we established stable RAW264.7 cell lines with Trem2 knockdown using lentivirus-mediated shRNA, followed by IL-4–induced polarization. Tubular epithelial fragments were prepared from TCMK-1 cells subjected to hypoxia/reoxygenation (H/R) and subsequently co-cultured with macrophages. RNA sequencing was performed to compare transcriptional profiles between normal Arg1⁺ macrophages and Trem2-deficient Arg1⁺ macrophages under tubular debris stimulation.2026/04/13GSE306859GSM9210935GSM9210934GSM9210933GSM9210932GSM9210931GSM921093034290306859Mus musculus[42039193]