{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Methylation profiling"],"species":["Homo sapiens"],"gds_type":["Methylation profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307137"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Spaceflight multi-omics reveals vulnerabilities of human germ cell development","description":"Exposure to microgravity, cosmic radiation, and other spaceflight conditions induces significant physiological adaptations in biological systems. While prior studies have characterized effects on stem cell self-renewal and differentiation, the biological impacts of spaceflight on human germ cell development remain unexplored. To address this gap, we deployed automated experimental platforms aboard China's Tianzhou-1 (TZ-1) and Tianzhou-6 spacecraft, enabling fluorescent morphological analysis and multi-omics profiling of stem cell derivatives under orbital conditions. These systems incorporated live-cell imaging, remote operation interfaces from Earth, and integrated sample preservation for time-point-specific specimen capture. Our experiments revealed that spaceflight significantly reduced germ cell yields and dysregulated translational profiles, particularly in cytoskeletal organization and extracellular matrix (ECM) interaction pathways. However, whole-exome sequencing and genome-wide methylation analyses confirmed genomic stability, with no statistically significant mutations during short-term spaceflight. This work establishes an automated platform for real-time analysis of human germ cell differentiation in orbital microgravity and provides a foundation for future space biology research.","dates":{"publication":"2026/06/03"},"accession":"GSE307137","cross_references":{"GSM":["GSM9217112","GSM9217113","GSM9217116","GSM9217117","GSM9217114","GSM9217115"],"GPL":["24676"],"GSE":["307137"],"taxon":["Homo sapiens"]}}