GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307225/primary200OKOtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307225GEOGSEfalseUltra-sensitive profiling of CRISPR-Cas off-target effects with Tracking-seq2Accurate detection of off-target activity in patient-derived cells is critical for ensuring the safety of gene therapeutics. Here, we present Tracking-seq2—the most sensitive, cell-based, and clinically applicable off-target detection technology to date, capable of profiling diverse genome editors. Crucially, we reveal that genomic variations drive distinct off-target heterogeneity across different cells, highlighting the necessity for personalized safety assessment in clinical genome editing therapies2026/06/16GSE307225GSM9716215GSM9716216GSM9716213GSM9716214GSM9716219GSM9716217GSM9716218GSM9219419GSM9219418GSM9219417GSM9219412GSM9219411GSM9219410GSM9716211GSM9219416GSM9219415GSM9716212GSM9219414GSM9716210GSM9219413GSM9716204GSM9716205GSM9716202GSM9716203GSM9716208GSM9716209GSM9716206GSM9716207GSM9219409GSM9219408GSM9219407GSM9219406GSM9219401GSM9219400GSM9716200GSM9219405GSM9716201GSM9219404GSM9219403GSM9219402GSM9716237GSM9716238GSM9716235GSM9716236GSM9219430GSM9716239GSM9219439GSM9219434GSM9716230GSM9219433GSM9219432GSM9219431GSM9716233GSM9219438GSM9219437GSM9716234GSM9716231GSM9219436GSM9219435GSM9716232GSM9716226GSM9716227GSM9716224GSM9716225GSM9716228GSM9716229GSM9219429GSM9219428GSM9219423GSM9219422GSM9219421GSM9219420GSM9219427GSM9716222GSM9219426GSM9716223GSM9716220GSM9219425GSM9716221GSM9219424GSM9716138GSM9716259GSM9716139GSM9716136GSM9716257GSM9716137GSM9716258GSM9716251GSM9716252GSM9716250GSM9716255GSM9716256GSM9716253GSM9716254GSM9716248GSM9716249GSM9716246GSM9716247GSM9716240GSM9716241GSM9716244GSM9716245GSM9716242GSM9716243GSM9716158GSM9716159GSM9716270GSM9716273GSM9716152GSM9716153GSM9716274GSM9716150GSM9716271GSM9716151GSM9716272GSM9716156GSM9716157GSM9716154GSM9716155GSM9716149GSM9716147GSM9716268GSM9716148GSM9716269GSM9716141GSM9716262GSM9716142GSM9716263GSM9716260GSM9716140GSM9716261GSM9716145GSM9716266GSM9716267GSM9716146GSM9716264GSM9716143GSM9716144GSM9716265GSM9716170GSM9716171GSM9716174GSM9716175GSM9716172GSM9716173GSM9716178GSM9716179GSM9716176GSM9716177GSM9219481GSM9219480GSM9716169GSM9219482GSM9716160GSM9716163GSM9716164GSM9716161GSM9716162GSM9716167GSM9716168GSM9716165GSM9716166GSM9219393GSM9219392GSM9219391GSM9219390GSM9219397GSM9219396GSM9219395GSM9219394GSM9716192GSM9716193GSM9716190GSM9716191GSM9716196GSM9716197GSM9716194GSM9219399GSM9219398GSM9716195GSM9716198GSM9716199GSM9716181GSM9716182GSM9716180GSM9716185GSM9219389GSM9716186GSM9716183GSM9716184GSM9716189GSM9716187GSM9716188GSM9219452GSM9219451GSM9219450GSM9219456GSM9219455GSM9219454GSM9219453GSM9219459GSM9219458GSM9219457GSM9219441GSM9219440GSM9219445GSM9219444GSM9219443GSM9219442GSM9219449GSM9219448GSM9219447GSM9219446GSM9219470GSM9219474GSM9219473GSM9219472GSM9219471GSM9219478GSM9219477GSM9219476GSM9219475GSM9219479GSM9219463GSM9219462GSM9219461GSM9219460GSM9219467GSM9219466GSM9219465GSM9219464GSM9219469GSM921946829480307225Homo sapiens