GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307308/primaryOK200TranscriptomicsOryctolagus cuniculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307308GEOGSEfalseSingle-cell RNA-Seq analysis of rabbit filtering bleb conjunctivaTo investigate changes in fibroblasts in tissues after glaucoma filtration surgery (GFS) using mitomycin C (MMC), we performed scRNA-Seq on bleb-conjunctival tissues after GFS using MMC. We successfully sequenced 4349, 3683, 2588, and 2921 single cells from rabbit GFS tissue of control (without surgery), 3 hours, 3 days, and 14 days after surgery, respectively. An unbiased clustering of single cells projected onto a UMAP space identified 21 major clusters. On days 3 and 14 after surgery, the number of identified fibroblasts was reduced to less than 30% compared to the control group, suggesting the effect of MMC on inducing cell death in fibroblasts. Thus, SASP-related factors such as CDKN1A, IL6, CXCL8, CCL2, and CCL7 were upregulated in fibroblasts after surgery.2026/08/26GSE307308GSM9221629GSM9221627GSM9221628GSM922163035700307308Oryctolagus cuniculus[42625400]