{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307777/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307777"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Hydrogel-Encapsulated Primed Mesenchymal Stem Cells Enhance Regeneration and Immune Modulation in Full-Thickness Burn Wounds","description":"Severe burn injuries pose significant clinical challenges, with high risks of infection, excessive inflammation, and impaired wound healing. Mesenchymal stem cells (MSCs) have shown regenerative and antimicrobial potential; however, their therapeutic efficacy is constrained by poor survival and engraftment. Here, we demonstrate that priming MSCs with insulin-secreting cells (ISCs) and encapsulating them in hydrogels (HEMI) enhances their regenerative function, leading to accelerated healing of full-thickness burns in a porcine model. Wounds treated with HEMIs achieved 80% closure within five weeks, compared to 50% in MSC-only and 0% in standard-of-care controls. Histological analysis revealed complete epidermal and dermal regeneration with minimal fibrosis in HEMI-treated wounds. Single-cell RNA sequencing (scRNA-seq) and differentially abundant sequencing (DA-seq) analysis identified distinct MSC subpopulations transitioning from a proliferative to a tissue-remodeling phenotype, with insulin priming promoting pathways involved in extracellular matrix (ECM) stabilization, immune modulation, and oxidative stress resistance. Our findings suggest that insulin priming enhances MSC-mediated tissue repair via paracrine mechanisms, providing a clinically translatable strategy for improving burn treatment and regenerative medicine applications.","dates":{"publication":"2026/06/30"},"accession":"GSE307777","cross_references":{"GSM":["GSM9231217"],"GPL":["34284"],"GSE":["307777"],"taxon":["Homo sapiens"]}}