{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307999/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":[" Expression profiling by high throughput sequencing","Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307999"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA and TCR sequencing of tumor-infiltrating lymphocytes and draining lymph node immune cells following AMP-410 treatment","description":"We employed single-cell RNA sequencing (scRNA-seq) and single-cell TCR sequencing (scTCR-seq) using the 10X Genomics platform to investigate immune cell dynamics during AMP-410 treatment in MC38 tumor-bearing b-h4-1BB transgenic mice. AMP-410 is a bifunctional VEGF-4-1BB biologic designed to simultaneously target VEGF and 4-1BB pathways, delivering a localized and synergistic approach to cancer immunotherapy. This study demonstrates that dual targeting of VEGF and 4-1BB signaling normalizes the tumor microenvironment, prevents T cell exhaustion in the TME in the later stage and also reduce the T cell conal exchange between tumor microenvironment and draining lymph nodes (dLNs), suggesting a local activity.","dates":{"publication":"2026/09/10"},"accession":"GSE307999","cross_references":{"GSM":["GSM9235386","GSM9235396","GSM9235385","GSM9235388","GSM9235387","GSM9235393","GSM9235392","GSM9235395","GSM9235394","GSM9235391","GSM9235390","GSM9235389"],"GPL":["24247"],"GSE":["307999"],"taxon":["Mus musculus"]}}