GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE307nnn/GSE307999/primaryOK200OtherMus musculus Expression profiling by high throughput sequencingOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE307999GEOGSEfalseSingle-cell RNA and TCR sequencing of tumor-infiltrating lymphocytes and draining lymph node immune cells following AMP-410 treatmentWe employed single-cell RNA sequencing (scRNA-seq) and single-cell TCR sequencing (scTCR-seq) using the 10X Genomics platform to investigate immune cell dynamics during AMP-410 treatment in MC38 tumor-bearing b-h4-1BB transgenic mice. AMP-410 is a bifunctional VEGF-4-1BB biologic designed to simultaneously target VEGF and 4-1BB pathways, delivering a localized and synergistic approach to cancer immunotherapy. This study demonstrates that dual targeting of VEGF and 4-1BB signaling normalizes the tumor microenvironment, prevents T cell exhaustion in the TME in the later stage and also reduce the T cell conal exchange between tumor microenvironment and draining lymph nodes (dLNs), suggesting a local activity.2026/09/10GSE307999GSM9235386GSM9235396GSM9235385GSM9235388GSM9235387GSM9235393GSM9235392GSM9235395GSM9235394GSM9235391GSM9235390GSM923538924247307999Mus musculus