{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308274/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308274"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq analysis of wild-type H-18 ESCs and EpiLCs","description":"Female BVSC clone H18 mESCs were cultured in 2i+LIF culture medium (N2B27 Media, CHIR99021 [30 µM; NC9785126; Thermo Fisher], PD0325901 [10 µM; NC9753132; Thermo Fisher], ESGRO Leukemia Inhibitory Factor (LIF) [1000 U/mL, ESG1106; Sigma-Aldrich]) for 72 hr. Differentiation of ESCs to EpiLCs was performed by seeding the cells on Human Plasma Fibronectin (HPF)-coated plates [16.7 µg/mL; 33016015; Thermo Fisher] in the presence of EpiLC induction medium (N2B27 medium containing activin A [20 ng/mL; 50-398-465; Thermo Fisher]), basic fibroblast growth factor (bFGF) [12 ng/mL; 3139FB025; R&D Systems], and KnockOut Serum Replacement [KSR, 1%; Thermo Fisher]. EpiLC samples were collected 48 hours after starting differentiation. Samples were mock treated with water.","dates":{"publication":"2026/05/27"},"accession":"GSE308274","cross_references":{"GSM":["GSM9241515","GSM9241516","GSM9241513","GSM9241514","GSM9241517","GSM9241512"],"GPL":["34290"],"GSE":["308274"],"taxon":["Mus musculus"],"PMID":["[41442442]"]}}