{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308307/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308307"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Integration of the naïve pluripotency gene network with the response to Gsk3 inhibition by Tcf7l1-driven enhancer decommissioning (Microarray)","description":"The transition out of naïve pluripotency requires the simultaneous dissolution of the naïve gene regulatory network as well as a redirection of transcriptional response to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at Tcf7l1-bound cis-regulatory elements in mouse pluripotent stem cells. A combination of gene knockout and inducible expression lines of ESC were generated to experimentally isolate effects of Tcf/Lef factors on pluripotency. Naïve cis-regulatory elements were specifically bound by and rapidly decommissioned by Tcf7l1 upon releasing cells from Gsk3 inhibition. These findings suggest that Tcf7l1-mediated enhancer decommissioning serves as a critical mechanism by which pluripotent cells rapidly alter the response to signaling pathways in preparation for gastrulation, and it may be a mechanism used in diverse cell types for modulating effects of Wnt/β-catenin signaling pathway on cell fate decisions.","dates":{"publication":"2026/07/29"},"accession":"GSE308307","cross_references":{"GSM":["GSM9241980","GSM9241981","GSM9241984","GSM9241962","GSM9241985","GSM9241963","GSM9241982","GSM9241960","GSM9241961","GSM9241983","GSM9241999","GSM9241977","GSM9241978","GSM9241975","GSM9241997","GSM9241998","GSM9241976","GSM9241959","GSM9241979","GSM9242002","GSM9241991","GSM9241970","GSM9242003","GSM9241992","GSM9242000","GSM9242001","GSM9241990","GSM9241973","GSM9241995","GSM9241996","GSM9241974","GSM9242004","GSM9241993","GSM9241971","GSM9241972","GSM9241994","GSM9242005","GSM9241988","GSM9241966","GSM9241967","GSM9241989","GSM9241964","GSM9241986","GSM9241987","GSM9241965","GSM9241968","GSM9241969"],"GPL":["6885"],"GSE":["308307"],"taxon":["Mus musculus"],"PMID":["[42392086]"]}}