GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308307/primary200OKTranscriptomicsMus musculusExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308307GEOGSEfalseIntegration of the naïve pluripotency gene network with the response to Gsk3 inhibition by Tcf7l1-driven enhancer decommissioning (Microarray)The transition out of naïve pluripotency requires the simultaneous dissolution of the naïve gene regulatory network as well as a redirection of transcriptional response to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at Tcf7l1-bound cis-regulatory elements in mouse pluripotent stem cells. A combination of gene knockout and inducible expression lines of ESC were generated to experimentally isolate effects of Tcf/Lef factors on pluripotency. Naïve cis-regulatory elements were specifically bound by and rapidly decommissioned by Tcf7l1 upon releasing cells from Gsk3 inhibition. These findings suggest that Tcf7l1-mediated enhancer decommissioning serves as a critical mechanism by which pluripotent cells rapidly alter the response to signaling pathways in preparation for gastrulation, and it may be a mechanism used in diverse cell types for modulating effects of Wnt/β-catenin signaling pathway on cell fate decisions.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 musculus[42392086]