{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308359/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"," Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308359"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RPL41 stabilizes ribosome dynamics and supports long-protein homeostasis in mammals","description":"Ribosomal protein L41 (RPL41 or eL41) is the smallest ribosomal protein and forms the eukaryote-specific bridge, eB14, near the decoding center; however, its role in mammalian translation remains unclear. In this study, we established RPL41-deficient models of human HEK293T cells and mice to define its function. Cryo-electron microscopy revealed that RPL41 constrains intersubunit conformational dynamics without inducing major local static rearrangements. Loss of RPL41 altered A-site dynamics, slowed elongation, modestly increased amino acid misincorporation, and modestly enhanced readthrough of collision-inducing reporter sequences. Quantitative proteomic analysis suggested that these translational defects compromise long-protein homeostasis, as evidenced by increased insolubility and reduced abundance of long proteins. In vivo, Rpl41−/− mice were viable but exhibited growth retardation and decreased abundance of long proteins in tissues. Our findings reveal a conserved role for RPL41 in maintaining ribosome dynamics and translational fidelity, indicating that RPL41 supports ribosome function and long-protein homeostasis in mammals.","dates":{"publication":"2026/07/27"},"accession":"GSE308359","cross_references":{"GSM":["GSM9244012","GSM9244011","GSM9244010","GSM9244016","GSM9244015","GSM9244014","GSM9244013","GSM9244019","GSM9244018","GSM9244017","GSM9243999","GSM9244023","GSM9244022","GSM9244021","GSM9244020","GSM9244027","GSM9243972","GSM9243971","GSM9244026","GSM9243970","GSM9244025","GSM9244024","GSM9243976","GSM9243975","GSM9244029","GSM9243974","GSM9243973","GSM9244028","GSM9243969","GSM9243968","GSM9244030","GSM9244034","GSM9244033","GSM9244032","GSM9244031","GSM9243983","GSM9244038","GSM9243982","GSM9244037","GSM9244036","GSM9243981","GSM9243980","GSM9244035","GSM9243987","GSM9243986","GSM9243985","GSM9244039","GSM9243984","GSM9243979","GSM9243978","GSM9243977","GSM9244041","GSM9244040","GSM9243990","GSM9244001","GSM9244000","GSM9243994","GSM9244005","GSM9243993","GSM9244004","GSM9244003","GSM9243992","GSM9243991","GSM9244002","GSM9244009","GSM9243998","GSM9244008","GSM9243997","GSM9243996","GSM9244007","GSM9244006","GSM9243995","GSM9243989","GSM9243988"],"GPL":["24676","24247"],"GSE":["308359"],"taxon":["Mus musculus"," Homo sapiens"],"PMID":["[42613035]"]}}