GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308359/primaryOK200Transcriptomics Mus musculusHomo sapiens OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308359GEOGSEfalseRPL41 stabilizes ribosome dynamics and supports long-protein homeostasis in mammalsRibosomal protein L41 (RPL41 or eL41) is the smallest ribosomal protein and forms the eukaryote-specific bridge, eB14, near the decoding center; however, its role in mammalian translation remains unclear. In this study, we established RPL41-deficient models of human HEK293T cells and mice to define its function. Cryo-electron microscopy revealed that RPL41 constrains intersubunit conformational dynamics without inducing major local static rearrangements. Loss of RPL41 altered A-site dynamics, slowed elongation, modestly increased amino acid misincorporation, and modestly enhanced readthrough of collision-inducing reporter sequences. Quantitative proteomic analysis suggested that these translational defects compromise long-protein homeostasis, as evidenced by increased insolubility and reduced abundance of long proteins. In vivo, Rpl41−/− mice were viable but exhibited growth retardation and decreased abundance of long proteins in tissues. Our findings reveal a conserved role for RPL41 in maintaining ribosome dynamics and translational fidelity, indicating that RPL41 supports ribosome function and long-protein homeostasis in mammals.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 Mus musculusHomo sapiens[42613035]