GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308431/primaryOK200TranscriptomicsRattus norvegicusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308431GEOGSEfalseRNA-seq analysis of spiral ganglion neurons treated with cisplatin and dimethyl fumarate to elucidate otoprotective mechanismsThis study aims to elucidate the potential protective mechanisms of dimethyl fumarate (DMF) against cisplatin-induced ototoxicity at the transcriptomic level. Spiral ganglion neurons (SGNs) were divided into two treatment groups: cisplatin alone and cisplatin combined with DMF. Gene expression profiling was performed using high-throughput RNA-sequencing. Differential expression analysis identified a set of significantly upregulated and downregulated genes in the DMF combination group compared to the cisplatin-only group. Pathway enrichment analysis highlighted significant alterations in autophagy and mitophagy pathways, suggesting that DMF may alleviate cisplatin-induced damage by modulating these processes in SGNs. This dataset provides the raw sequencing reads (FASTQ) and gene count matrix, serving as a valuable resource for investigating drug-induced ototoxicity and protective therapeutic strategies.2026/09/15GSE308431GSM9245348GSM9245349GSM9245344GSM9245345GSM9245346GSM924534725947308431Rattus norvegicus