{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308432/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308432"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Activation of donor-derived eosinophils contributes to primary graft dysfunction during static lung graft storage","description":"To explore the impact of antioxidant supplementation on eosinophil transcriptional responses during lung preservation, we performed bulk RNA sequencing on purified eosinophils cultured in either standard media (RPMI) or Perfadex® Plus, with or without glutathione (GSH). Cells were incubated at 37°C to mimic rewarming conditions that typically induce activation. Exposure to Perfadex® Plus resulted in reduced cell viability and suppression of eosinophil-associated transcriptional programs related to recruitment, activation, and survival. In contrast, supplementation with GSH preserved cell viability and significantly enhanced the expression of antioxidant and redox-related pathways. Enrichment analysis revealed activation of oxidative stress response, detoxification, glutathione metabolism, selenium-associated processes, and mitochondrial respiration. These findings suggest that GSH mitigates metabolic stress and dampens pro-inflammatory transcriptional activity in eosinophils, supporting its potential role in improving lung preservation outcomes.","dates":{"publication":"2026/09/17"},"accession":"GSE308432","cross_references":{"GSM":["GSM9245351","GSM9245352","GSM9245353","GSM9245354","GSM9245350","GSM9245355","GSM9245356","GSM9245357","GSM9245358"],"GPL":["21103"],"GSE":["308432"],"taxon":["Mus musculus"]}}