{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308448/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308448"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Gene expression profiling of HBEpCs and Caco-2 cells during infection with WT SARS-CoV-2 or a recombinant SARS-CoV-2 deficient in PLpro deISGylation activity","description":"Interferon-stimulated gene 15 (ISG15) regulates diverse cellular processes, including antiviral immunity, through its conjugation to proteins, a process known as ISGylation. Several pathogens, including SARS-CoV-2, subvert ISGylation by encoding deISGylating enzymes. However, the direct targets and physiological consequences of coronaviral deISGylation remain poorly defined. Here, we engineered a recombinant SARS-CoV-2 harboring a mutant Nsp3 (Nsp3mut) with impaired papain-like protease (PLpro) deISGylating activity. Caco-2 and human bronchial epithelial (HBEpC) cells were infected with either wild-type or Nsp3mut SARS-CoV-2. Comparative transcriptomic profiling revealed that Nsp3mut infection led to a stronger induction of IFN- and ISG-related gene signatures compared to wild-type virus infection in both cell types. These results show that loss of PLpro-mediated deISGylation restores efficient antiviral innate immune responses during SARS-CoV-2 infection.","dates":{"publication":"2026/05/08"},"accession":"GSE308448","cross_references":{"GSM":["GSM9245722","GSM9245733","GSM9245723","GSM9245734","GSM9245735","GSM9245724","GSM9245725","GSM9245736","GSM9245740","GSM9245730","GSM9245741","GSM9245742","GSM9245731","GSM9245732","GSM9245726","GSM9245737","GSM9245727","GSM9245738","GSM9245739","GSM9245728","GSM9245729"],"GPL":["34284"],"GSE":["308448"],"taxon":["Homo sapiens"]}}