GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308541/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308541GEOGSEfalseTranscriptomic effects of miR-21-5p on preimplantation mouse blastocystsPreimplantation embryos cultured in vitro exhibit increased stress and apoptosis compared with in vivo development. miR-21-5p is a conserved, highly abundant microRNA in reproductive-tract extracellular vesicles and has been implicated in cell-survival pathways. To define its transcriptomic impact on early embryos, we performed RNA-seq on mouse blastocysts generated by IVF and cultured in KSOM with either miR-21-5p agomir (50 nM) or blank control. Each biological replicate consisted of five pooled blastocysts; n=4 replicates per group. Sequencing was carried out on an Illumina NovaSeq X Plus.2026/06/15GSE308541GSM9247782GSM9247783GSM9247780GSM9247781GSM9247779GSM9247776GSM9247777GSM924777834290308541Mus musculus