{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308594/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308594"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"BoNT/A Modulates Microglial Phagocytic Activity in the Hippocampus Revealed by Single-Cell RNA Sequencing","description":"To investigate the impact of Botulinum Neurotoxin A (BoNT/A) on hippocampal cellular landscapes, we performed single-cell RNA sequencing (scRNA-seq) on tissues from Saline-, MPTP-, and MPTP+BoNT/A-treated mice. We identified and annotated 10 major cell types through canonical marker gene expression. A focused sub-clustering analysis of microglia revealed 11 distinct subpopulations. Notably, KEGG pathway enrichment analysis identified three subclusters (1, 3, and 5) as phagocytosis-related microglia, enriched in pathways such as lysosome, phagosome, and FcγR-mediated phagocytosis. Our findings demonstrate that BoNT/A plays a definitive role in modulating microglial phagocytic activity in the hippocampus.","dates":{"publication":"2026/06/24"},"accession":"GSE308594","cross_references":{"GSM":["GSM9248946","GSM9248945","GSM9248947"],"GPL":["34328"],"GSE":["308594"],"taxon":["Mus musculus"],"PMID":["[42263400]"]}}