GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308594/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308594GEOGSEfalseBoNT/A Modulates Microglial Phagocytic Activity in the Hippocampus Revealed by Single-Cell RNA SequencingTo investigate the impact of Botulinum Neurotoxin A (BoNT/A) on hippocampal cellular landscapes, we performed single-cell RNA sequencing (scRNA-seq) on tissues from Saline-, MPTP-, and MPTP+BoNT/A-treated mice. We identified and annotated 10 major cell types through canonical marker gene expression. A focused sub-clustering analysis of microglia revealed 11 distinct subpopulations. Notably, KEGG pathway enrichment analysis identified three subclusters (1, 3, and 5) as phagocytosis-related microglia, enriched in pathways such as lysosome, phagosome, and FcγR-mediated phagocytosis. Our findings demonstrate that BoNT/A plays a definitive role in modulating microglial phagocytic activity in the hippocampus.2026/06/24GSE308594GSM9248946GSM9248945GSM924894734328308594Mus musculus[42263400]