{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308718/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308718"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Regenerative macrophages enhance stem cell-derived beta-cell function and engraftment","description":"The generation of insulin-producing beta cells from human embryonic stem cells (SC-β cells) holds promise for treating type 1 diabetes. Transplantation of SC-β cells is already in clinical testing, but generating mature cells with insulin-secreting properties similar to endogenous cells has been challenging. Since macrophages are essential for islet development, we hypothesized they could enhance SC-β-cell differentiation and function. We co-aggregated SC-macrophages which were either unpolarized (SC-MUnp), or polarized to inflammatory (SC-MInf) or regenerative (SC-MReg) states during stage 7 of SC-β-cell differentiation. SC-MRegs improved maturity marker expression, glucose-stimulated insulin secretion, and metabolic activity in SC-β cells. Transplantation of SC-β cells co-aggregated with SC-MRegs into diabetic mice normalized glycemia significantly faster than transplantation of SC-β cells alone. The finding that addition of macrophages during SC-β differentiation accelerates functional maturation represents a significant advance in the production of SC-β cells as a regenerative cell therapy for type 1 diabetes.","dates":{"publication":"2026/06/12"},"accession":"GSE308718","cross_references":{"GSM":["GSM9251997","GSM9251998","GSM9251999","GSM9252000","GSM9252001","GSM9252002"],"GPL":["24676"],"GSE":["308718"],"taxon":["Homo sapiens"]}}