GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308764/primaryOK200TranscriptomicsXenopus laevisExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308764GEOGSEfalseConserved roles of GATA4 and its target gene TBX2 in regulation of human cardiogenesisThe transcription factor (TF) GATA4 is a key mediator of cardiogenesis. GATA4 regulates cardiogenesis through the expression of its target genes, only some of which have been identified. We have used a gain of function model based on pluripotent embryonic ectoderm explants from Xenopus embryos expressing GATA4, to identify a set of downstream targets of GATA4 which are also regulated by Nodal, a known cardiogenic signal. GATA4 was shown to be required for the expression of target genes tbx2 and prdm1 in vivo, likely acting in a direct fashion by interacting with their regulatory regions. In addition, tbx2 and prdm1 are shown to have roles of their own in vivo, as downregulation of tbx2, a positive target, and overexpression of prdm1, a negative target, interferes with cardiac development in Xenopus embryos.2026/04/29GSE308764GSM9252755GSM9252756GSM9252757GSM9252758GSM9252747GSM9252748GSM9252759GSM9252749GSM9252760GSM9252761GSM9252750GSM9252751GSM9252762GSM9252752GSM9252753GSM925275418936308764Xenopus laevis