GEOTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309395GEOGSEfalseExtracellular vesicle-derived circRNA profiles from cART suppressed pregnant women living with HIV identifies interactome networks key to inflammation and viral latencyPregnant people living with HIV (PLWH) experience a disparate risk of chronic non-AIDS-related morbidities (NAMs) during pregnancy compared to people without HIV infection. Extracellular vesicles (EV) are fundamental to both HIV pathogenesis and maternal-placental-fetal systemic intercellular networking. However, there is limited research addressing the intersection of EV cargo and HIV during pregnancy. In this study, plasma-derived EV small RNA (smRNA) in pregnant PLWH as compared to pregnant people living without HIV (PLWoH) was examined through low-input RNA sequencing (Illumina PE150). Differential expression analysis identified significant alterations of EV circRNA levels for pregnant PLWH as compared to pregnant PLWoH. Novel interactome networks (circRNA-miRNA-gene and circRNA-protein) were generated from these differentially expressed EV circRNAs. Gene set enrichment analysis revealed physiological pathways influential to HIV reservoir establishment, latency, replication, immune activation, immunosenescence, as well as maternal-placental-fetal immune cross talk. These findings suggest that EV circRNA-mediated regulatory mechanisms may contribute to HIV persistence, inflammation, and immune modulation in pregnancy.2026/06/03GSE309395GSM9266799GSM9266810GSM9266800GSM9266811GSM9266812GSM9266801GSM9266802GSM9266813GSM9266803GSM9266814GSM9266815GSM9266804GSM9266805GSM9266816GSM9266806GSM9266807GSM9266808GSM926680920795309395Homo sapiens[42231329]