{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE309nnn/GSE309709/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309709"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Three immunoregulatory signatures define non-productive HIV infection in CD4+ T memory stem cells","description":"The persistent HIV reservoir constitutes the main obstacle to curing HIV/AIDS disease. Our understanding of how non-productive HIV infections are established in primary human CD4+ T cells during the first round of infection remains, however, incomplete. In this study, we leveraged the HIV reporter virus pMorpheus-V5 to delineate cellular expression patterns that are upregulated in non-productively infected primary CD4+ T memory stem cells (TSCM). We found that CD4+ TSCM harboring non-productive proviruses displayed a distinct transcriptomic signature comprising 118 upregulated genes. This non-productive expression profile was distinct from that of productively infected cells as well as from negative-exposed and mock-infected cells. Among the cellular genes most upregulated in CD4+ T cells harboring non-productive proviruses were CCR4-binding migratory chemokines (CCL22 and CCL17), tryptophan catabolic enzymes (IDO1 and KYNU), and genes encoding cytoskeletal rearrangement proteins (BASP1 and TNFAIP2). Intra-cellular FACS-based analyses revealed that non-productively infected CD4+ TSCM cells were enriched (i.e., 8.3%) for CCL22 and IDO1 co-expression compared to the other CD4+ memory subsets, underscoring a clear CD4+ T cell subset specificity for the upregulation of these two immune gene sets associated with non-productive infections. These findings suggest that primary human CD4+ TSCMharboring non-productive proviruses display a distinct immunoregulatory phenotype.","dates":{"publication":"2026/05/15"},"accession":"GSE309709","cross_references":{"GSM":["GSM9277812","GSM9277823","GSM9277822","GSM9277811","GSM9277821","GSM9277820","GSM9277819","GSM9277818","GSM9277817","GSM9277816","GSM9277826","GSM9277815","GSM9277814","GSM9277825","GSM9277824","GSM9277813"],"GPL":["34281"],"GSE":["309709"],"taxon":["Homo sapiens"]}}