{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309916"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Feeder-free generation of functional dendritic cells from human pluripotent stem cells","description":"The scarcity of primary conventional dendritic cells (cDCs) and the limited effectiveness of monocyte-derived dendritic cells (moDCs) have long hindered progress in human dendritic cell research and immunotherapy. We developed a feeder-free differentiation platform that generates CD1c⁺CD141⁺ hPSC-cDCs phenotypically aligned with CD141⁺ tissue-resident cDC2 subsets found in human tissues. We further optimized the differentiation process using a Design-of-Experiments framework to refine cytokine and serum conditions, enhancing differentiation efficiency while reducing cytokine demand. These hPSC-cDCs exhibit efficient antigen uptake, defined cytokine responses, and robust priming of antigen-specific CD8⁺ T cell proliferation and effector differentiation, outperforming moDCs in direct comparison. Together, this work establishes a robust, and generalizable platform for mechanistic studies and translational development of dendritic cell-based vaccines and standardized ex vivo T cell expansion.","dates":{"publication":"2026/05/13"},"accession":"GSE309916","cross_references":{"GSM":["GSM9284782","GSM9284781","GSM9284790","GSM9284786","GSM9284785","GSM9284784","GSM9284783","GSM9284789","GSM9284788","GSM9284787"],"GPL":["34281"],"GSE":["309916"],"taxon":["Homo sapiens"]}}