{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE309993"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effector differentiation by stem-like intraepithelial γδ T cells is required for host defense against infection","description":"Intraepithelial γδ T cells (γδIELs) are crucial for maintaining intestinal homeostasis, yet their functional regulation remains incompletely understood. Here, we identified two distinct γδIEL subsets: CD160+TCF1+BCL6+ stem-like and granzymeB+BLIMP1+ effector-like cells; the latter exhibited cytotoxic activity. Stem-like γδIELs differentiated into effector-like cells in response to microbiota and pathogen infection. IL-12, in cooperation with IL-15 and TCR signaling, activated a STAT4-BLIMP1 pathway to induce effector differentiation, and IL-12 signaling was required for their cytotoxic function. Tcf7 deletion significantly reduced stem-like but not effector-like γδIELs, while Bcl6 deficiency increased effector-like γδIELs selectively. In contrast, the absence of Prdm1 not only abolished the development of effector-like γδIELs but also impaired the clearance of enterogenous pathogens. Taken together, these findings define two functionally distinct γδIEL subsets with a hierarchical regulatory program linking microbial signals and cytokine pathways to their cytotoxic function in the gut.","dates":{"publication":"2026/04/13"},"accession":"GSE309993","cross_references":{"GSM":["GSM9286206","GSM9286207","GSM9286208","GSM9286209","GSM9286198","GSM9286210","GSM9286199","GSM9286211","GSM9286194","GSM9286195","GSM9286196","GSM9286197","GSM9286190","GSM9286191","GSM9286192","GSM9286193","GSM9286202","GSM9286203","GSM9286204","GSM9286205","GSM9286187","GSM9286188","GSM9286189","GSM9286200","GSM9286201"],"GPL":["28457","24247"],"GSE":["309993"],"taxon":["Mus musculus"],"PMID":["[41747734]"]}}