{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310138/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310138"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Sex-Biased Transcription in Early Postnatal Layer 2/3 Cortical Neurons: Interplay Between Sex and Cux1/2 Mutations","description":"Despite many neurodevelopmental disorders (NDDs) exhibiting pronounced sex differences, the role of sex in shaping the genetic regulation of the brain remains barely explored. Brain development relies on a precise control of gene expression across time and disruptions in this process, especially during critical periods, can result in NDDs. In the neocortex, layer (L) 2/3 excitatory pyramidal neurons stand out for integrating information across cortical areas and hemispheres. Here, we show a transient window of sex-biased gene expression of L2/3 neurons at postnatal day 4 (P4), mainly characterized by autosomal genes. Loss-of-function experiments targeting Cux1 and Cux2, key transcription factors in L2/3 development, lead to sex-dependent transcriptional deregulation in these neurons, with a significant male-biased sensitivity. Moreover, differentially expressed genes shift substantially between developmental stages, reflecting the dynamic regulation of Cux-mediated gene expression. Overall, our study identifies P4 as a key determinant of early sex differences and highly vulnerable stage for the emergence of NDDs, and highlights the importance of sex-specific analyses for understanding brain development and disease.","dates":{"publication":"2026/05/20"},"accession":"GSE310138","cross_references":{"GSM":["GSM9289418","GSM9289419","GSM9289405","GSM9289406","GSM9289403","GSM9289404","GSM9289401","GSM9289402","GSM9289388","GSM9289389","GSM9289400","GSM9289386","GSM9289387","GSM9289384","GSM9289385","GSM9289382","GSM9289383","GSM9289380","GSM9289381","GSM9289390","GSM9289409","GSM9289407","GSM9289408","GSM9289416","GSM9289417","GSM9289414","GSM9289415","GSM9289412","GSM9289379","GSM9289413","GSM9289399","GSM9289410","GSM9289377","GSM9289378","GSM9289411","GSM9289397","GSM9289398","GSM9289395","GSM9289396","GSM9289393","GSM9289394","GSM9289391","GSM9289392"],"GPL":["30172"],"GSE":["310138"],"taxon":["Mus musculus"],"PMID":["[41952201]"]}}