{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310189/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310189"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Thermoneutral housing augments T cell receptor (TCR)-mediated CD4+ T cell responsiveness","description":"Mice (Mus musculus) used in biomedical research are commonly housed at 19-23 °C, below their thermoneutral zone (29-34 °C), where metabolic homeostasis occurs. Although traditional housing exposes mice to chronic cold stress and modulates their immune response, cellular mechanisms by which thermoneutrality shapes immune responses remain underdefined. CD4+ T cells are major contributors to host immunity through T cell receptor (TCR) signaling activated via recognition of peptide-major histocompatibility complex class II (pMHCII). We demonstrate that thermoneutral housing, compared to traditional conditions, enhances basal TCR signaling, upregulates genes associated with endogenous TCR stimulation, and increases TCR-driven TNF expression in CD4⁺ T cells. Mechanistically, these effects are in part dependent on basal TCR engagement with self-pMHCII. In inflammatory disease models, thermoneutrality-driven increased CD4⁺ T cell TNF production correlates with amplified tissue inflammation. Together, these findings reveal how housing temperature shapes inflammatory responses through self-pMHCII-dependent TCR signaling in CD4⁺ T cells.","dates":{"publication":"2026/03/31"},"accession":"GSE310189","cross_references":{"GSM":["GSM9290780","GSM9290781","GSM9290770","GSM9290779","GSM9290768","GSM9290769","GSM9290782","GSM9290771","GSM9290772","GSM9290773","GSM9290774","GSM9290775","GSM9290776","GSM9290777","GSM9290778"],"GPL":["30172"],"GSE":["310189"],"taxon":["Mus musculus"]}}