{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310322/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310322"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Mapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted T cell fate","description":"This study aims to define how Id2 and Id3 regulate exhausted CD8+ T cell fate. We found that Id2 deletion reduced the Tex-eff population, whereas Id3 ablation in CD4-Cre mice diminished Tpex cells, indicating subset-specific roles for Id2 and Id3. Mechanistically, E2A is a major target of Id2/3, and Runx3 interacts with E2A. Pulldown of Id2/3 recovered both E2A and Runx3, supporting an Id2/3?E2A?Runx3 regulatory complex. To map the genomic occupancy of Id2 and Id3, we expressed 3×T7-tagged Id2 or Id3 in in vitro-primed CD45.2+ P14 cells, adoptively transferred these cells into CD45.1 recipient mice, and infected the recipients with LCMV Clone 13. On day 6 post-infection, Tex-eff and Tpex cells were isolated for T7 CUT&RUN to define Id2 binding in Tex-eff cells and Id3 binding in Tpex cells, with the empty T7 vector backbone used as a negative control.","dates":{"publication":"2026/04/20"},"accession":"GSE310322","cross_references":{"GSM":["GSM9613816","GSM9613817","GSM9613818","GSM9293980","GSM9293982","GSM9293981","GSM9293995","GSM9293994","GSM9293997","GSM9293996","GSM9293977","GSM9293999","GSM9293998","GSM9293979","GSM9293978","GSM9608237","GSM9608238","GSM9294000","GSM9608239","GSM9293991","GSM9293990","GSM9293993","GSM9293992","GSM9613820","GSM9293984","GSM9293983","GSM9293986","GSM9293985","GSM9608232","GSM9293988","GSM9613819","GSM9608233","GSM9608234","GSM9293987","GSM9608235","GSM9293989","GSM9608236"],"GPL":["24247"],"GSE":["310322"],"taxon":["Mus musculus"]}}