GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310322/primaryOK200GenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310322GEOGSEfalseMapping self-associating chromatin hubs identifies Id proteins as key determinants of exhausted T cell fateThis study aims to define how Id2 and Id3 regulate exhausted CD8+ T cell fate. We found that Id2 deletion reduced the Tex-eff population, whereas Id3 ablation in CD4-Cre mice diminished Tpex cells, indicating subset-specific roles for Id2 and Id3. Mechanistically, E2A is a major target of Id2/3, and Runx3 interacts with E2A. Pulldown of Id2/3 recovered both E2A and Runx3, supporting an Id2/3?E2A?Runx3 regulatory complex. To map the genomic occupancy of Id2 and Id3, we expressed 3×T7-tagged Id2 or Id3 in in vitro-primed CD45.2+ P14 cells, adoptively transferred these cells into CD45.1 recipient mice, and infected the recipients with LCMV Clone 13. On day 6 post-infection, Tex-eff and Tpex cells were isolated for T7 CUT&RUN to define Id2 binding in Tex-eff cells and Id3 binding in Tpex cells, with the empty T7 vector backbone used as a negative control.2026/04/20GSE310322GSM9613816GSM9613817GSM9613818GSM9293980GSM9293982GSM9293981GSM9293995GSM9293994GSM9293997GSM9293996GSM9293977GSM9293999GSM9293998GSM9293979GSM9293978GSM9608237GSM9608238GSM9294000GSM9608239GSM9293991GSM9293990GSM9293993GSM9293992GSM9613820GSM9293984GSM9293983GSM9293986GSM9293985GSM9608232GSM9293988GSM9613819GSM9608233GSM9608234GSM9293987GSM9608235GSM9293989GSM960823624247310322Mus musculus