{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310695/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"," Rattus norvegicus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310695"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Systematic Benchmarking of CUT&Tag Improves the Reliability and Reproducibility of Chromatin Analysis [Cell number]","description":"Cleavage under target and tagementation (CUT&Tag) is a widely used assay for analyzing epigenomic localization of proteins and histone PTMs. This study is focused on increasing the reproducibility of CUT&Tag through standardization of both wet bench and downstream data analysis. We find that 10K cells is the minimum input requirement for robust genome-wide profiling of H3K7me3 in mESCs and rat C6 cells. For CTCF 50K cells are needed. EGS crosslinking and de-duplication strategies were also explored.","dates":{"publication":"2026/07/16"},"accession":"GSE310695","cross_references":{"GSM":["GSM9307113","GSM9307079","GSM9307112","GSM9307115","GSM9307114","GSM9307117","GSM9307116","GSM9307119","GSM9307118","GSM9307070","GSM9307072","GSM9307071","GSM9307074","GSM9307073","GSM9307076","GSM9307075","GSM9307078","GSM9307111","GSM9307110","GSM9307077","GSM9307124","GSM9307123","GSM9307126","GSM9307125","GSM9307128","GSM9307127","GSM9307129","GSM9307081","GSM9307080","GSM9307083","GSM9307082","GSM9307085","GSM9307084","GSM9307087","GSM9307120","GSM9307086","GSM9307122","GSM9307089","GSM9307121","GSM9307088","GSM9307090","GSM9307092","GSM9307091","GSM9307094","GSM9307093","GSM9307096","GSM9307095","GSM9307098","GSM9307131","GSM9307130","GSM9307097","GSM9307133","GSM9307099","GSM9307132","GSM9307102","GSM9307069","GSM9307101","GSM9307068","GSM9307104","GSM9307103","GSM9307106","GSM9307105","GSM9307108","GSM9307107","GSM9307109","GSM9307065","GSM9307064","GSM9307067","GSM9307100","GSM9307066"],"GPL":["34316","34290"],"GSE":["310695"],"taxon":["Mus musculus"," Rattus norvegicus"],"PMID":["[42285100]"]}}