GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310696/primaryOK200GenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310696GEOGSEfalseSystematic Benchmarking of CUT&Tag Improves the Reliability and Reproducibility of Chromatin Analysis [Crosslinking]Cleavage under target and tagementation (CUT&Tag) is a widely used assay for analyzing epigenomic localization of proteins and histone PTMs. This study is focused on increasing the reproducibility of CUT&Tag through standardization of both wet bench and downstream data analysis. We find that 10K cells is the minimum input requirement for robust genome-wide profiling of H3K7me3 in mESCs and rat C6 cells. For CTCF 50K cells are needed. EGS crosslinking and de-duplication strategies were also explored.2026/07/16GSE310696GSM9307157GSM9307135GSM9307134GSM9307156GSM9307137GSM9307159GSM9307158GSM9307136GSM9307139GSM9307138GSM9307151GSM9307150GSM9307153GSM9307152GSM9307155GSM9307154GSM9307146GSM9307145GSM9307148GSM9307147GSM9307149GSM9307160GSM9307140GSM9307162GSM9307161GSM9307164GSM9307142GSM9307163GSM9307141GSM9307144GSM930714334284310696Homo sapiens