{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310875/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Bos taurus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310875"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Low oxygen environment alters transcripts related to energy metabolism without altering the pluripotency core of bovine embryonic stem cells","description":"Here we evaluated effects of the adapation of bovine embryonic stem cells (bESC) previously derived on mouse embryonic fibroblasts (MEF) under high oxygen (21%) conditions in low oxygen environment (5%) and their their molecular profile. Primed bESC established on MEF were adapted to 5% O2 conditions after 5-6 passages. Following culture in low oxygen for 5 passages, bESC were transitioned to feeder-free conditions. Comparing the transcriptomes of bESC cultured in , we found that adaptation to feeder-free conditions triggered commitment towards cell differentiation. Transition of bESC on MEF to a low oxygen environment did not result in changes in morphology or growth. Furthermore, we noted fewer changes in the transcriptome of cells grown on MEF compared to those grown in feeder-free conditions when maintained in low oxygen, compared to cells maintained in atmospheric oxygen. Lastly, while low oxygen tension did not alter the pluripotency profile of bESC, it did promote changes in transcripts related to energetic metabolism. These findings enhance our understanding about the optimal conditions for bESC culture and emphasize the importance of oxygen tension for derivation, maintenance, and performance of embryonic stem cells.","dates":{"publication":"2026/06/10"},"accession":"GSE310875","cross_references":{"GSM":["GSM9310655","GSM9310656","GSM9310657","GSM9310658","GSM9310652","GSM9310653","GSM9310654","GSM9310659"],"GPL":["23295"],"GSE":["310875"],"taxon":["Bos taurus"],"PMID":["[42051204]"]}}