{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310967/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Saccharomyces cerevisiae"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310967"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Mapping intasome integration sites and nucleosome dyads in chromatinized yeast DNA","description":"HIV-1 and other lentiviruses hijack the cellular chromatin-binding protein LEDGF/p75 to facilitate integration into active transcription units. However, the mechanism of chromatin engagement by lentiviral intasomes and the structural role of LEDGF/p75 in this process remain poorly understood. To address these gaps, we studied the activities of in vitro-assembled retroviral intasomes in the presence of chromatinized target DNA. While LEDGF/p75 was both essential and minimally sufficient to enhance lentiviral integration into chromatin containing histone H3 trimethylated on Lys36, it unexpectedly facilitated integration outside of the nucleosome core particles. LEDGF/p75 additionally inhibited integration into unmodified chromatin in a dose-dependent manner, promoting integration into naked DNA. The results establish the preference of lentiviral intasomes for linker DNA regions within H3K36Me3-enriched chromatin.","dates":{"publication":"2026/07/13"},"accession":"GSE310967","cross_references":{"GSM":["GSM9313417","GSM9313416","GSM9313419","GSM9313418","GSM9313413","GSM9313412","GSM9313415","GSM9313414","GSM9313431","GSM9313430","GSM9313433","GSM9313411","GSM9313432","GSM9313410","GSM9313409","GSM9313406","GSM9313428","GSM9313427","GSM9313405","GSM9313408","GSM9313429","GSM9313407","GSM9313424","GSM9313423","GSM9313426","GSM9313404","GSM9313403","GSM9313425","GSM9313420","GSM9313422","GSM9313421"],"GPL":["25739","36344"],"GSE":["310967"],"taxon":["Saccharomyces cerevisiae"]}}