GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE310nnn/GSE310967/primaryOK200OtherSaccharomyces cerevisiaeOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE310967GEOGSEfalseMapping intasome integration sites and nucleosome dyads in chromatinized yeast DNAHIV-1 and other lentiviruses hijack the cellular chromatin-binding protein LEDGF/p75 to facilitate integration into active transcription units. However, the mechanism of chromatin engagement by lentiviral intasomes and the structural role of LEDGF/p75 in this process remain poorly understood. To address these gaps, we studied the activities of in vitro-assembled retroviral intasomes in the presence of chromatinized target DNA. While LEDGF/p75 was both essential and minimally sufficient to enhance lentiviral integration into chromatin containing histone H3 trimethylated on Lys36, it unexpectedly facilitated integration outside of the nucleosome core particles. LEDGF/p75 additionally inhibited integration into unmodified chromatin in a dose-dependent manner, promoting integration into naked DNA. The results establish the preference of lentiviral intasomes for linker DNA regions within H3K36Me3-enriched chromatin.2026/07/13GSE310967GSM9313417GSM9313416GSM9313419GSM9313418GSM9313413GSM9313412GSM9313415GSM9313414GSM9313431GSM9313430GSM9313433GSM9313411GSM9313432GSM9313410GSM9313409GSM9313406GSM9313428GSM9313427GSM9313405GSM9313408GSM9313429GSM9313407GSM9313424GSM9313423GSM9313426GSM9313404GSM9313403GSM9313425GSM9313420GSM9313422GSM93134212573936344310967Saccharomyces cerevisiae