GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311042/primaryOK200TranscriptomicsSaccharomyces cerevisiaeExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311042GEOGSEfalseSuppression of upstream ORF translation is not the primary mechanism for translational stimulation by yeast helicase Ded1Impairment of DEAD-box RNA helicase Ded1 function results in substantial reduction in translational efficiencies (TEs) of main open reading frames (mORFs) in budding yeast. However, it was unknown whether this translation reduction in ded1 mutants is brought about by the increased translation of upstream open reading frames (uORFs).To examine this, we combined ribosome footprint profiling with RNA-seq analysis of mRNA abundance and determined the relative changes in TEs of uORFs and mORFs in ded1 mutants versus wild-type cells.2026/05/15GSE311042GSM9315904GSM9315905GSM9315902GSM9315903GSM9315900GSM9315901GSM9315898GSM9315910GSM9315899GSM9315896GSM9315897GSM9315895GSM9315908GSM9315909GSM9315906GSM931590717342311042Saccharomyces cerevisiae[42087789]