{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311208/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311208"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Combination of single-cell and bulk RNA-seq reveals changes in the immune landscape in osteomyelitis","description":"Objective: This study profiled the osteomyelitis immune micro-environment in depth, pinpointed driver genes and cell populations that fuel disease progression, and mined the data for actionable drug targets. Methods: We analyzed time-series transcriptomic sequencing data from mouse tibial osteomyelitis samples in dataset GSE168896. Fuzzy c-means clustering was applied to reveal gene sets linked to disease progression. Immune cell infiltration analysis was conducted through online tool ImmuCellAI-mouse. Furthermore, by leveraging single-cell sequencing data, we characterized immune cell subpopulations and pinpointed the key cell subtypes that exhibited in osteomyelitis mouse. Results: We identified six gene clusters exhibiting distinct temporal expression patterns and functional roles in osteomyelitis processes, such as leukocyte and lymphocyte activation, ossification. Single-cell sequencing analysis further revealed 7 distinct cellular subpopulations. Among these, M2-like macrophages demonstrated a significant increase following osteomyelitis. Arg+Sdc4+ Mac and Cxcl1+Ccl4+ Mac were new subpopulations of macrophages that emerged after osteomyelitis, the infiltration of Mif+Cd63+ Mac significantly increased. Besides, Cxcl2-Cxcr2 ligand-receptors contributed mostly in immune cells.","dates":{"publication":"2026/02/25"},"accession":"GSE311208","cross_references":{"GSM":["GSM9321145","GSM9321143","GSM9321144"],"GPL":["19057"],"GSE":["311208"],"taxon":["Mus musculus"],"PMID":["[12982112]"]}}