GEOTranscriptomicsOvis ariesExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311245GEOGSEfalseTranscriptome of primed and naïve-like state sheep embryonic stem cellsThis project investigates the transcriptional profiles of sheep embryonic stem cells (sESCs) maintained in different culture conditions required for the maintenance of distinct pluripotent states. We derived primed-state sESCs from in vivo blastocysts using a chemically defined culture system and characterized their morphology and molecular features. When cultured in defined media previously shown to stabilize naïve pluripotency in human ESCs, sESCs adopted dome-shaped colony morphology, and exhibited features consistent with a naïve-like state. RNA sequencing was performed on both primed and naïve-like sESCs to define gene expression differences, signaling pathway changes, and regulatory networks associated with pluripotency state transitions in sheep.2026/06/01GSE311245GSM9321943GSM9321944GSM9321941GSM9321942GSM9321947GSM9321948GSM9321945GSM932194636106311245Ovis aries