GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311457/primaryOK200TranscriptomicsRhodopseudomonas palustrisExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311457GEOGSEfalseUsing Rhodopseudomonas palustris as a model bacterium to study fungal-bacterial interactions in wood decayed by the brown rot fungus Rhodonia placentaBrown rot fungi play an essential role in carbon cycling by decomposing lignocellulose into substrates usable by themselves and other microbes. Interactions between bacteria and fungi can be competitive or beneficial, but these relationships are not well understood because of a lack of good model systems. To model cross-feeding between fungi and bacteria, wood decayed by the brown rot fungus Rhodonia placenta was used as a carbon source for the phototrophic bacterium Rhodopseudomonas palustris. We found that fungal decay products generated by Rda. placenta could be used by R. palustris for growth, and later decay stages contained more usable substrates than early stages. Mass spectrometry identified a range of aromatic and non-aromatic acids, but after 95 days of bacterial growth, R. palustris only consumed non-aromatic acids over the aromatic lignin monomers. Genes involved with aromatic compound degradation were unimportant for growth and RNA sequencing revealed that aromatic compound degradation genes were repressed on decayed wood extract. Randomly barcoded transposon sequencing failed to identify a solitary catabolic pathway used by R. palustris, suggestive of substrate co-utilization, and surprisingly showed that genes involved with copper toxicity were essential. Finally, we found genes involved with biosynthesis of certain cofactors were non-essential on decayed wood extract, suggesting these nutrients were scavenged, and implicating these resources in supporting microbial communities in the environment. This study helps lay the foundation for a model fungal-bacterial system to study interactions that govern these relationships which will be valuable for understanding ecological interactions and industrially relevant co-culture systems.2026/06/08GSE311457GSM9325541GSM9325542GSM9325543GSM932554436042311457Rhodopseudomonas palustris