GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311487/primary200OKTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311487GEOGSEfalseThe TENT5A–ATXN2 axis modulates cellular survival during heat stress.Stress conditions elicit the formation of different kinds of stress granules. The assembly and composition of heat-induced stress granules remain poorly characterized. Here, we show that the cytoplasmic RNA polyadenylases TENT5A regulate the formation and function of ATXN2-positive heat-induced stress granules. Tent5a mRNA expression increases when somatic and germ cells are exposed to temperatures between 40°C and 42°C, but not upon exposure to other stressors. TENT5A decreases ATXN2 levels by reducing Atxn2 mRNA translation and accelerating ATXN2 protein decay. Upon heat exposure of the testis, ATXN2-positive stress granules are formed in pre-meiotic and meiotic cells independently of the presence of G3BP1 and the heat stress response protein DAZL. Depletion of TENT5A leads to increased cell-type-specific formation of ATXN2-positive stress granules and differentially regulates cell survival. In sum, TENT5A inhibits the formation of ATXN2-positive granules to modulate the heat shock response in vivo.2026/07/10GSE311487GSM9325936GSM932593724973311487Mus musculus