{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311792/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311792"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A patient-specific lung tumoroid platform recapitulates tumor microenvironments for modeling in vitro therapeutic responses and immune-stromal interactions","description":"Understanding tumor–stroma–immune interactions is crucial for overcoming resistance to chemo-immunotherapy in non-small cell lung cancer (NSCLC), yet modeling the tumor microenvironment (TME) ex vivo remains challenging. Here, we developed patient-specific lung cancer tumoroid (LCT) models integrating TME by reassembling lung cancer organoids (LCOs), cancer-associated fibroblasts (CAFs) and immune cells, which isolated and expanded from tumor tissues by using a modular Transwell-based culture system. LCTs retained major TME components and spatial organization of the primary tumor. The system supported high-fidelity pharmacologic screening, immune profiling, and mechanistic analysis of treatment-induced TME remodeling. Using LCTs, we observed tumor-intrinsic variation in immune engagement, CAF-mediated modulation, and an uncoupling between metabolic activation and immune stimulation. The LCT enables functional interrogation of patient-specific stromal–immune–tumor interactions, provides mechanistic insights into chemo-immunotherapy resistance, and offers a clinically relevant tool for precision immune-oncology and organoid-based therapeutic modeling.","dates":{"publication":"2026/09/09"},"accession":"GSE311792","cross_references":{"GSM":["GSM9332245","GSM9332234","GSM9332233","GSM9332244","GSM9332247","GSM9332236","GSM9332246","GSM9332235","GSM9332249","GSM9332238","GSM9332248","GSM9332237","GSM9332239","GSM9332250","GSM9332252","GSM9332230","GSM9332241","GSM9332251","GSM9332240","GSM9332243","GSM9332232","GSM9332231","GSM9332242","GSM9332253"],"GPL":["24676"],"GSE":["311792"],"taxon":["Homo sapiens"],"PMID":["[42316214]"]}}