{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311803/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Arabidopsis thaliana"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311803"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Root meristem growth factor (RGF) peptide signaling as a molecular bridge between root development and non-lethal thermal stress adaptation","description":"Roots adapt to temperature ranges that restrict growth without causing lethality, a largely unexplored physiological state. Although lethal heat shock and moderately high temperatures have been studied in detail, the effects of non-lethal high temperatures on root development remain largely unknown. We defined 31°C as a non-lethal thermal stress in Arabidopsis thaliana and examined its impact on root growth using phenotypic analyses and developmental-zone-specific transcriptomics. Under the non-lethal thermal stress (31°C), primary root growth, meristem size, and superoxide (O₂-) accumulation were all reduced, and the distribution of the meristem master regulator PLETHORA2 (PLT2) became restricted. Transcriptome analysis revealed a strong downregulation of RGFs, RGFRs, and PLT2. Mutants of these genes were more sensitive to the stress, whereas RGF1 treatment restored meristem size, increased O₂- levels, and expanded the distribution of PLT2 protein. RGF peptide also promoted lateral root development, resulting in a more complex root system under the thermal stress. These results indicate that the RGF-RGF receptor-PLT2 pathway plays a central role in root adaptation to the non-lethal heat stress and suggest that manipulating RGF signaling could enhance root thermotolerance and crop resilience under elevated temperatures.","dates":{"publication":"2026/06/14"},"accession":"GSE311803","cross_references":{"GSM":["GSM9332355","GSM9332366","GSM9332365","GSM9332354","GSM9332368","GSM9332357","GSM9332367","GSM9332356","GSM9332359","GSM9332369","GSM9332358","GSM9332360","GSM9332370","GSM9332362","GSM9332351","GSM9332361","GSM9332353","GSM9332364","GSM9332363","GSM9332352"],"GPL":["30821"],"GSE":["311803"],"taxon":["Arabidopsis thaliana"]}}