{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311971/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311971"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of lineage reprogramming by dynamic chromatin SUMOylation (ChIP-seq)","description":"SUMOylation has emerged as a key regulator of chromatin and transcription, yet its contribution to lineage reprogramming remains unclear. To explore how chromatin SUMOylation influences cellular plasticity, we studied CEBPA-driven lineage reprogramming of human leukemic B-cells into macrophage-like cells. By integrating ChIP-seq, ATAC-seq, RNA-seq and chromatin-directed proteomics, we mapped the chromatin landscape and transcriptomic changes during early reprogramming. Lineage conversion triggered a dynamic rise in SUMO2/3 chromatin occupancy at CEBPA-bound sites, revealing a coordinated regulatory mechanism. Proteomic profiling of SUMO2/3- and CEBPA-associated chromatin uncovered extensive convergence and enrichment of differentiation-related transcription factors, chromatin remodelers and coregulators. Among these, NCOA3 displayed markedly increased SUMO2/3 association upon lineage conversion. NCOA3 co-occupied CEBPA- and SUMO2/3-bound chromatin regions, implying a SUMOylation-supported coregulatory role in lineage reprogramming. Pharmacological inhibition of SUMOylation using ML-792 (SUMOi) selectively enhanced CEBPA chromatin occupancy and chromatin accessibility, altered the CEBPA association of proteins, and modified NCOA3 binding dynamics. SUMOi also reshaped gene expression, promoting loss of B-cell identity and activation of macrophage-associated programs, including lipid metabolism. Collectively, our findings highlight chromatin SUMOylation as a dynamic and context-dependent modifier that fine-tunes lineage transitions, with implications for chromatin biology and therapeutic modulation of cell identity.","dates":{"publication":"2026/05/27"},"accession":"GSE311971","cross_references":{"GSM":["GSM9335403","GSM9335404","GSM9335401","GSM9335423","GSM9335424","GSM9335402","GSM9335407","GSM9335408","GSM9335405","GSM9335406","GSM9335384","GSM9335385","GSM9335421","GSM9335388","GSM9335389","GSM9335422","GSM9335400","GSM9335386","GSM9335387","GSM9335420","GSM9335414","GSM9335415","GSM9335412","GSM9335413","GSM9335418","GSM9335419","GSM9335416","GSM9335417","GSM9335395","GSM9335396","GSM9335393","GSM9335394","GSM9335410","GSM9335399","GSM9335411","GSM9335397","GSM9335398","GSM9335391","GSM9335392","GSM9335390","GSM9335409"],"GPL":["11154"],"GSE":["311971"],"taxon":["Homo sapiens"]}}