{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311975/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311975"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of lineage reprogramming by dynamic chromatin SUMOylation (RNA-seq)","description":"SUMOylation has emerged as a key regulator of chromatin and transcription, yet its contribution to lineage reprogramming remains unclear. To explore how chromatin SUMOylation influences cellular plasticity, we studied CEBPA-driven lineage reprogramming of human leukemic B-cells into macrophage-like cells. By integrating ChIP-seq, ATAC-seq, RNA-seq and chromatin-directed proteomics, we mapped the chromatin landscape and transcriptomic changes during early reprogramming. Lineage conversion triggered a dynamic rise in SUMO2/3 chromatin occupancy at CEBPA-bound sites, revealing a coordinated regulatory mechanism. Proteomic profiling of SUMO2/3- and CEBPA-associated chromatin uncovered extensive convergence and enrichment of differentiation-related transcription factors, chromatin remodelers and coregulators. Among these, NCOA3 displayed markedly increased SUMO2/3 association upon lineage conversion. NCOA3 co-occupied CEBPA- and SUMO2/3-bound chromatin regions, implying a SUMOylation-supported coregulatory role in lineage reprogramming. Pharmacological inhibition of SUMOylation using ML-792 (SUMOi) selectively enhanced CEBPA chromatin occupancy and chromatin accessibility, altered the CEBPA association of proteins, and modified NCOA3 binding dynamics. SUMOi also reshaped gene expression, promoting loss of B-cell identity and activation of macrophage-associated programs, including lipid metabolism. Collectively, our findings highlight chromatin SUMOylation as a dynamic and context-dependent modifier that fine-tunes lineage transitions, with implications for chromatin biology and therapeutic modulation of cell identity.","dates":{"publication":"2026/05/27"},"accession":"GSE311975","cross_references":{"GSM":["GSM9335469","GSM9335502","GSM9335503","GSM9335500","GSM9335489","GSM9335467","GSM9335501","GSM9335468","GSM9335504","GSM9335505","GSM9335483","GSM9335484","GSM9335481","GSM9335482","GSM9335487","GSM9335465","GSM9335466","GSM9335488","GSM9335485","GSM9335486","GSM9335464","GSM9335480","GSM9335478","GSM9335479","GSM9335494","GSM9335472","GSM9335495","GSM9335473","GSM9335492","GSM9335470","GSM9335471","GSM9335493","GSM9335498","GSM9335476","GSM9335477","GSM9335499","GSM9335474","GSM9335496","GSM9335497","GSM9335475","GSM9335490","GSM9335491"],"GPL":["11154"],"GSE":["311975"],"taxon":["Homo sapiens"]}}