GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312338/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312338GEOGSEfalseTranscriptional profiling of estrogen-responsive uterine genes in Rptorf/f Pgrcre/+ miceTo investigate the molecular mechanisms by which mTORC1 signaling inactivation contributes to thin endometrium, we performed uterine transcriptomic profiling in ovariectomized Rptorf/f Pgrcre/+ mice (n=3) and Rptorf/f control (n=3) mice 24 hours after estrogen stimulation. RNA was extracted with TRIzol, and quality-confirmed (A260/280 >1.8, RIN >8.0). Stranded mRNA libraries were prepared (TruSeq Stranded mRNA LT Kit) and sequenced on Illumina Novaseq 6000. Reads were aligned to GRCm38.p6 (HISAT2), and gene expression was quantified as FPKM (Cufflinks) and read counts (HTSeq-count).2026/05/15GSE312338GSM9343901GSM9343902GSM9343903GSM9343904GSM9343905GSM934390624247312338Mus musculus[41826809]