{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312358/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312358"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Multi-omics Analysis of Immune Cells Induced by Artificial Adjuvant Vector Cell (aAVC) Therapy in HPV-Positive Tumor-Bearing Mice [Spatial Transcriptomics]","description":"Immune evasion by tumors highlights the need to activate both CTLs and NK cells against “immune-desert” type of tumor, yet the synergistic potential of dual activation in immune-desert tumors remains unclear. Here, we uncover a spatiotemporal synergy between NK cells and CTLs that drives functional antitumor effects in an immune-desert tumor model. Using a multi-omics imaging approach integrating spatial transcriptomics, scRNA-seq re-clustering, and multicolor flow cytometry, we show that vaccination rapidly reshapes lymphocyte localization and function in the tumor microenvironment (TME). Early activated IRF8+Klrg1+NK cell infiltration establishes a niche that guides subsequent antigen-primed CXCR3+CTL influx, accompanied by dendritic cell activation and inflammatory monocyte recruitment. This coordinated response relies on structural and chemotactic remodeling of the TME, facilitating NK-CTL interactions at the tumor margin. Our findings demonstrate how integrated multi-omics analyses can uncover previously inaccessible interactions and provide a framework for designing next-generation immunotherapies.","dates":{"publication":"2026/06/21"},"accession":"GSE312358","cross_references":{"GSM":["GSM9344297","GSM9344298"],"GPL":["33896"],"GSE":["312358"],"taxon":["Mus musculus"]}}