{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312516/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Danio rerio"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312516"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Wnt–β-catenin signaling promotes zebrafish osteoblast dedifferentiation by wnt10a-mediated inhibition of NF-κB","description":"Dedifferentiation of mature cells is a key mechanism for generating source cells in many regenerative systems, including the zebrafish fin. Here we show that osteoblast injury responses involve rapid and extensive transcriptional reprogramming, yielding a cell state that closely resembles embryonic osteoblasts while also activating regeneration-specific genes. Among these, wnt10a is the only canonical Wnt ligand induced specifically in osteoblasts during early phases after amputation. Using genetic perturbations, we demonstrate that Wnt10a-dependent Wnt/β-catenin signaling is essential for osteoblast dedifferentiation and acts cell-autonomously to drive this process. Loss of wnt10a or Wnt/β-catenin activity blocks the dedifferentiation program, whereas wnt10a overexpression enhances dedifferentiation and is sufficient to induce it even without injury. Wnt/β-catenin signaling promotes dedifferentiation by suppressing NF-κB activity, placing it upstream of known inhibitory cues. Notably, wnt10a also stimulates cardiomyocyte dedifferentiation, revealing a conserved role in activating source-cell formation during regeneration.","dates":{"publication":"2026/10/01"},"accession":"GSE312516","cross_references":{"GSM":["GSM9347690","GSM9347708","GSM9347707","GSM9347709","GSM9347711","GSM9347699","GSM9347710","GSM9347713","GSM9347712","GSM9347715","GSM9347714","GSM9347717","GSM9347716","GSM9347692","GSM9347691","GSM9347694","GSM9347693","GSM9347696","GSM9347695","GSM9347698","GSM9347697","GSM9347719","GSM9347718","GSM9347722","GSM9347700","GSM9347689","GSM9347688","GSM9347721","GSM9347702","GSM9347724","GSM9347723","GSM9347701","GSM9347726","GSM9347704","GSM9347725","GSM9347703","GSM9347706","GSM9347705","GSM9347727","GSM9347681","GSM9347683","GSM9347682","GSM9347685","GSM9347684","GSM9347720","GSM9347687","GSM9347686"],"GPL":["24995"],"GSE":["312516"],"taxon":["Danio rerio"]}}