GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE312nnn/GSE312516/primaryOK200TranscriptomicsDanio rerioExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE312516GEOGSEfalseWnt–β-catenin signaling promotes zebrafish osteoblast dedifferentiation by wnt10a-mediated inhibition of NF-κBDedifferentiation of mature cells is a key mechanism for generating source cells in many regenerative systems, including the zebrafish fin. Here we show that osteoblast injury responses involve rapid and extensive transcriptional reprogramming, yielding a cell state that closely resembles embryonic osteoblasts while also activating regeneration-specific genes. Among these, wnt10a is the only canonical Wnt ligand induced specifically in osteoblasts during early phases after amputation. Using genetic perturbations, we demonstrate that Wnt10a-dependent Wnt/β-catenin signaling is essential for osteoblast dedifferentiation and acts cell-autonomously to drive this process. Loss of wnt10a or Wnt/β-catenin activity blocks the dedifferentiation program, whereas wnt10a overexpression enhances dedifferentiation and is sufficient to induce it even without injury. Wnt/β-catenin signaling promotes dedifferentiation by suppressing NF-κB activity, placing it upstream of known inhibitory cues. Notably, wnt10a also stimulates cardiomyocyte dedifferentiation, revealing a conserved role in activating source-cell formation during regeneration.2026/10/01GSE312516GSM9347690GSM9347708GSM9347707GSM9347709GSM9347711GSM9347699GSM9347710GSM9347713GSM9347712GSM9347715GSM9347714GSM9347717GSM9347716GSM9347692GSM9347691GSM9347694GSM9347693GSM9347696GSM9347695GSM9347698GSM9347697GSM9347719GSM9347718GSM9347722GSM9347700GSM9347689GSM9347688GSM9347721GSM9347702GSM9347724GSM9347723GSM9347701GSM9347726GSM9347704GSM9347725GSM9347703GSM9347706GSM9347705GSM9347727GSM9347681GSM9347683GSM9347682GSM9347685GSM9347684GSM9347720GSM9347687GSM934768624995312516Danio rerio